Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0226AhTE0226genetic_marker
AhTE0227AhTE0227genetic_marker
AhTE0228AhTE0228genetic_marker
AhTE0229AhTE0229genetic_marker
AhTE0230AhTE0230genetic_marker
AhTE0231AhTE0231genetic_marker
AhTE0232AhTE0232genetic_marker
AhTE0233AhTE0233genetic_marker
AhTE0234AhTE0234genetic_marker
AhTE0235AhTE0235genetic_marker
AhTE0236AhTE0236genetic_marker
AhTE0237AhTE0237genetic_marker
AhTE0238AhTE0238genetic_marker
AhTE0239AhTE0239genetic_marker
AhTE0240AhTE0240genetic_marker
AhTE0241AhTE0241genetic_marker
AhTE0242AhTE0242genetic_marker
AhTE0243AhTE0243genetic_marker
AhTE0244AhTE0244genetic_marker
AhTE0245AhTE0245genetic_marker
AhTE0246AhTE0246genetic_marker
AhTE0247AhTE0247genetic_marker
AhTE0248AhTE0248genetic_marker
AhTE0249AhTE0249genetic_marker
AhTE0250AhTE0250genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055