Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0201AhTE0201genetic_marker
AhTE0202AhTE0202genetic_marker
AhTE0203AhTE0203genetic_marker
AhTE0204AhTE0204genetic_marker
AhTE0205AhTE0205genetic_marker
AhTE0206AhTE0206genetic_marker
AhTE0207AhTE0207genetic_marker
AhTE0208AhTE0208genetic_marker
AhTE0209AhTE0209genetic_marker
AhTE0210AhTE0210genetic_marker
AhTE0211AhTE0211genetic_marker
AhTE0212AhTE0212genetic_marker
AhTE0213AhTE0213genetic_marker
AhTE0214AhTE0214genetic_marker
AhTE0215AhTE0215genetic_marker
AhTE0216AhTE0216genetic_marker
AhTE0217AhTE0217genetic_marker
AhTE0218AhTE0218genetic_marker
AhTE0219AhTE0219genetic_marker
AhTE0220AhTE0220genetic_marker
AhTE0221AhTE0221genetic_marker
AhTE0222AhTE0222genetic_marker
AhTE0223AhTE0223genetic_marker
AhTE0224AhTE0224genetic_marker
AhTE0225AhTE0225genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055