Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0176AhTE0176genetic_marker
AhTE0177AhTE0177genetic_marker
AhTE0178AhTE0178genetic_marker
AhTE0179AhTE0179genetic_marker
AhTE0180AhTE0180genetic_marker
AhTE0181AhTE0181genetic_marker
AhTE0182AhTE0182genetic_marker
AhTE0183AhTE0183genetic_marker
AhTE0184AhTE0184genetic_marker
AhTE0185AhTE0185genetic_marker
AhTE0186AhTE0186genetic_marker
AhTE0187AhTE0187genetic_marker
AhTE0188AhTE0188genetic_marker
AhTE0189AhTE0189genetic_marker
AhTE0190AhTE0190genetic_marker
AhTE0191AhTE0191genetic_marker
AhTE0192AhTE0192genetic_marker
AhTE0193AhTE0193genetic_marker
AhTE0194AhTE0194genetic_marker
AhTE0195AhTE0195genetic_marker
AhTE0196AhTE0196genetic_marker
AhTE0197AhTE0197genetic_marker
AhTE0198AhTE0198genetic_marker
AhTE0199AhTE0199genetic_marker
AhTE0200AhTE0200genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055