Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0151AhTE0151genetic_marker
AhTE0152AhTE0152genetic_marker
AhTE0153AhTE0153genetic_marker
AhTE0154AhTE0154genetic_marker
AhTE0155AhTE0155genetic_marker
AhTE0156AhTE0156genetic_marker
AhTE0157AhTE0157genetic_marker
AhTE0158AhTE0158genetic_marker
AhTE0159AhTE0159genetic_marker
AhTE0160AhTE0160genetic_marker
AhTE0161AhTE0161genetic_marker
AhTE0162AhTE0162genetic_marker
AhTE0163AhTE0163genetic_marker
AhTE0164AhTE0164genetic_marker
AhTE0165AhTE0165genetic_marker
AhTE0166AhTE0166genetic_marker
AhTE0167AhTE0167genetic_marker
AhTE0168AhTE0168genetic_marker
AhTE0169AhTE0169genetic_marker
AhTE0170AhTE0170genetic_marker
AhTE0171AhTE0171genetic_marker
AhTE0172AhTE0172genetic_marker
AhTE0173AhTE0173genetic_marker
AhTE0174AhTE0174genetic_marker
AhTE0175AhTE0175genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055