Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0126AhTE0126genetic_marker
AhTE0127AhTE0127genetic_marker
AhTE0128AhTE0128genetic_marker
AhTE0129AhTE0129genetic_marker
AhTE0130AhTE0130genetic_marker
AhTE0131AhTE0131genetic_marker
AhTE0132AhTE0132genetic_marker
AhTE0133AhTE0133genetic_marker
AhTE0134AhTE0134genetic_marker
AhTE0135AhTE0135genetic_marker
AhTE0136AhTE0136genetic_marker
AhTE0137AhTE0137genetic_marker
AhTE0138AhTE0138genetic_marker
AhTE0139AhTE0139genetic_marker
AhTE0140AhTE0140genetic_marker
AhTE0141AhTE0141genetic_marker
AhTE0142AhTE0142genetic_marker
AhTE0143AhTE0143genetic_marker
AhTE0144AhTE0144genetic_marker
AhTE0145AhTE0145genetic_marker
AhTE0146AhTE0146genetic_marker
AhTE0147AhTE0147genetic_marker
AhTE0148AhTE0148genetic_marker
AhTE0149AhTE0149genetic_marker
AhTE0150AhTE0150genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055