Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0101AhTE0101genetic_marker
AhTE0102AhTE0102genetic_marker
AhTE0103AhTE0103genetic_marker
AhTE0104AhTE0104genetic_marker
AhTE0105AhTE0105genetic_marker
AhTE0106AhTE0106genetic_marker
AhTE0107AhTE0107genetic_marker
AhTE0108AhTE0108genetic_marker
AhTE0109AhTE0109genetic_marker
AhTE0110AhTE0110genetic_marker
AhTE0111AhTE0111genetic_marker
AhTE0112AhTE0112genetic_marker
AhTE0113AhTE0113genetic_marker
AhTE0114AhTE0114genetic_marker
AhTE0115AhTE0115genetic_marker
AhTE0116AhTE0116genetic_marker
AhTE0117AhTE0117genetic_marker
AhTE0118AhTE0118genetic_marker
AhTE0119AhTE0119genetic_marker
AhTE0120AhTE0120genetic_marker
AhTE0121AhTE0121genetic_marker
AhTE0122AhTE0122genetic_marker
AhTE0123AhTE0123genetic_marker
AhTE0124AhTE0124genetic_marker
AhTE0125AhTE0125genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
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DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055