Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0076AhTE0076genetic_marker
AhTE0077AhTE0077genetic_marker
AhTE0078AhTE0078genetic_marker
AhTE0079AhTE0079genetic_marker
AhTE0080AhTE0080genetic_marker
AhTE0081AhTE0081genetic_marker
AhTE0082AhTE0082genetic_marker
AhTE0083AhTE0083genetic_marker
AhTE0084AhTE0084genetic_marker
AhTE0085AhTE0085genetic_marker
AhTE0086AhTE0086genetic_marker
AhTE0087AhTE0087genetic_marker
AhTE0088AhTE0088genetic_marker
AhTE0089AhTE0089genetic_marker
AhTE0090AhTE0090genetic_marker
AhTE0091AhTE0091genetic_marker
AhTE0092AhTE0092genetic_marker
AhTE0093AhTE0093genetic_marker
AhTE0094AhTE0094genetic_marker
AhTE0095AhTE0095genetic_marker
AhTE0096AhTE0096genetic_marker
AhTE0097AhTE0097genetic_marker
AhTE0098AhTE0098genetic_marker
AhTE0099AhTE0099genetic_marker
AhTE0100AhTE0100genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055