Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0051AhTE0051genetic_marker
AhTE0052AhTE0052genetic_marker
AhTE0053AhTE0053genetic_marker
AhTE0054AhTE0054genetic_marker
AhTE0055AhTE0055genetic_marker
AhTE0056AhTE0056genetic_marker
AhTE0057AhTE0057genetic_marker
AhTE0058AhTE0058genetic_marker
AhTE0059AhTE0059genetic_marker
AhTE0060AhTE0060genetic_marker
AhTE0061AhTE0061genetic_marker
AhTE0062AhTE0062genetic_marker
AhTE0063AhTE0063genetic_marker
AhTE0064AhTE0064genetic_marker
AhTE0065AhTE0065genetic_marker
AhTE0066AhTE0066genetic_marker
AhTE0067AhTE0067genetic_marker
AhTE0068AhTE0068genetic_marker
AhTE0069AhTE0069genetic_marker
AhTE0070AhTE0070genetic_marker
AhTE0071AhTE0071genetic_marker
AhTE0072AhTE0072genetic_marker
AhTE0073AhTE0073genetic_marker
AhTE0074AhTE0074genetic_marker
AhTE0075AhTE0075genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055