Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0476AhTE0476genetic_marker
AhTE0477AhTE0477genetic_marker
AhTE0478AhTE0478genetic_marker
AhTE0479AhTE0479genetic_marker
AhTE0480AhTE0480genetic_marker
AhTE0481AhTE0481genetic_marker
AhTE0482AhTE0482genetic_marker
AhTE0483AhTE0483genetic_marker
AhTE0484AhTE0484genetic_marker
AhTE0485AhTE0485genetic_marker
AhTE0486AhTE0486genetic_marker
AhTE0487AhTE0487genetic_marker
AhTE0488AhTE0488genetic_marker
AhTE0489AhTE0489genetic_marker
AhTE0490AhTE0490genetic_marker
AhTE0491AhTE0491genetic_marker
AhTE0492AhTE0492genetic_marker
AhTE0493AhTE0493genetic_marker
AhTE0494AhTE0494genetic_marker
AhTE0495AhTE0495genetic_marker
AhTE0496AhTE0496genetic_marker
AhTE0497AhTE0497genetic_marker
AhTE0498AhTE0498genetic_marker
AhTE0499AhTE0499genetic_marker
AhTE0500AhTE0500genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055