Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0451AhTE0451genetic_marker
AhTE0452AhTE0452genetic_marker
AhTE0453AhTE0453genetic_marker
AhTE0454AhTE0454genetic_marker
AhTE0455AhTE0455genetic_marker
AhTE0456AhTE0456genetic_marker
AhTE0457AhTE0457genetic_marker
AhTE0458AhTE0458genetic_marker
AhTE0459AhTE0459genetic_marker
AhTE0460AhTE0460genetic_marker
AhTE0461AhTE0461genetic_marker
AhTE0462AhTE0462genetic_marker
AhTE0463AhTE0463genetic_marker
AhTE0464AhTE0464genetic_marker
AhTE0465AhTE0465genetic_marker
AhTE0466AhTE0466genetic_marker
AhTE0467AhTE0467genetic_marker
AhTE0468AhTE0468genetic_marker
AhTE0469AhTE0469genetic_marker
AhTE0470AhTE0470genetic_marker
AhTE0471AhTE0471genetic_marker
AhTE0472AhTE0472genetic_marker
AhTE0473AhTE0473genetic_marker
AhTE0474AhTE0474genetic_marker
AhTE0475AhTE0475genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055