Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0426AhTE0426genetic_marker
AhTE0427AhTE0427genetic_marker
AhTE0428AhTE0428genetic_marker
AhTE0429AhTE0429genetic_marker
AhTE0430AhTE0430genetic_marker
AhTE0431AhTE0431genetic_marker
AhTE0432AhTE0432genetic_marker
AhTE0433AhTE0433genetic_marker
AhTE0434AhTE0434genetic_marker
AhTE0435AhTE0435genetic_marker
AhTE0436AhTE0436genetic_marker
AhTE0437AhTE0437genetic_marker
AhTE0438AhTE0438genetic_marker
AhTE0439AhTE0439genetic_marker
AhTE0440AhTE0440genetic_marker
AhTE0441AhTE0441genetic_marker
AhTE0442AhTE0442genetic_marker
AhTE0443AhTE0443genetic_marker
AhTE0444AhTE0444genetic_marker
AhTE0445AhTE0445genetic_marker
AhTE0446AhTE0446genetic_marker
AhTE0447AhTE0447genetic_marker
AhTE0448AhTE0448genetic_marker
AhTE0449AhTE0449genetic_marker
AhTE0450AhTE0450genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055