Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0401AhTE0401genetic_marker
AhTE0402AhTE0402genetic_marker
AhTE0403AhTE0403genetic_marker
AhTE0404AhTE0404genetic_marker
AhTE0405AhTE0405genetic_marker
AhTE0406AhTE0406genetic_marker
AhTE0407AhTE0407genetic_marker
AhTE0408AhTE0408genetic_marker
AhTE0409AhTE0409genetic_marker
AhTE0410AhTE0410genetic_marker
AhTE0411AhTE0411genetic_marker
AhTE0412AhTE0412genetic_marker
AhTE0413AhTE0413genetic_marker
AhTE0414AhTE0414genetic_marker
AhTE0415AhTE0415genetic_marker
AhTE0416AhTE0416genetic_marker
AhTE0417AhTE0417genetic_marker
AhTE0418AhTE0418genetic_marker
AhTE0419AhTE0419genetic_marker
AhTE0420AhTE0420genetic_marker
AhTE0421AhTE0421genetic_marker
AhTE0422AhTE0422genetic_marker
AhTE0423AhTE0423genetic_marker
AhTE0424AhTE0424genetic_marker
AhTE0425AhTE0425genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055