Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0376AhTE0376genetic_marker
AhTE0377AhTE0377genetic_marker
AhTE0378AhTE0378genetic_marker
AhTE0379AhTE0379genetic_marker
AhTE0380AhTE0380genetic_marker
AhTE0381AhTE0381genetic_marker
AhTE0382AhTE0382genetic_marker
AhTE0383AhTE0383genetic_marker
AhTE0384AhTE0384genetic_marker
AhTE0385AhTE0385genetic_marker
AhTE0386AhTE0386genetic_marker
AhTE0387AhTE0387genetic_marker
AhTE0388AhTE0388genetic_marker
AhTE0389AhTE0389genetic_marker
AhTE0390AhTE0390genetic_marker
AhTE0391AhTE0391genetic_marker
AhTE0392AhTE0392genetic_marker
AhTE0393AhTE0393genetic_marker
AhTE0394AhTE0394genetic_marker
AhTE0395AhTE0395genetic_marker
AhTE0396AhTE0396genetic_marker
AhTE0397AhTE0397genetic_marker
AhTE0398AhTE0398genetic_marker
AhTE0399AhTE0399genetic_marker
AhTE0400AhTE0400genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055