Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0351AhTE0351genetic_marker
AhTE0352AhTE0352genetic_marker
AhTE0353AhTE0353genetic_marker
AhTE0354AhTE0354genetic_marker
AhTE0355AhTE0355genetic_marker
AhTE0356AhTE0356genetic_marker
AhTE0357AhTE0357genetic_marker
AhTE0358AhTE0358genetic_marker
AhTE0359AhTE0359genetic_marker
AhTE0360AhTE0360genetic_marker
AhTE0361AhTE0361genetic_marker
AhTE0362AhTE0362genetic_marker
AhTE0363AhTE0363genetic_marker
AhTE0364AhTE0364genetic_marker
AhTE0365AhTE0365genetic_marker
AhTE0366AhTE0366genetic_marker
AhTE0367AhTE0367genetic_marker
AhTE0368AhTE0368genetic_marker
AhTE0369AhTE0369genetic_marker
AhTE0370AhTE0370genetic_marker
AhTE0371AhTE0371genetic_marker
AhTE0372AhTE0372genetic_marker
AhTE0373AhTE0373genetic_marker
AhTE0374AhTE0374genetic_marker
AhTE0375AhTE0375genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055