Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0326AhTE0326genetic_marker
AhTE0327AhTE0327genetic_marker
AhTE0328AhTE0328genetic_marker
AhTE0329AhTE0329genetic_marker
AhTE0330AhTE0330genetic_marker
AhTE0331AhTE0331genetic_marker
AhTE0332AhTE0332genetic_marker
AhTE0333AhTE0333genetic_marker
AhTE0334AhTE0334genetic_marker
AhTE0335AhTE0335genetic_marker
AhTE0336AhTE0336genetic_marker
AhTE0337AhTE0337genetic_marker
AhTE0338AhTE0338genetic_marker
AhTE0339AhTE0339genetic_marker
AhTE0340AhTE0340genetic_marker
AhTE0341AhTE0341genetic_marker
AhTE0342AhTE0342genetic_marker
AhTE0343AhTE0343genetic_marker
AhTE0344AhTE0344genetic_marker
AhTE0345AhTE0345genetic_marker
AhTE0346AhTE0346genetic_marker
AhTE0347AhTE0347genetic_marker
AhTE0348AhTE0348genetic_marker
AhTE0349AhTE0349genetic_marker
AhTE0350AhTE0350genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055