Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0301AhTE0301genetic_marker
AhTE0302AhTE0302genetic_marker
AhTE0303AhTE0303genetic_marker
AhTE0304AhTE0304genetic_marker
AhTE0305AhTE0305genetic_marker
AhTE0306AhTE0306genetic_marker
AhTE0307AhTE0307genetic_marker
AhTE0308AhTE0308genetic_marker
AhTE0309AhTE0309genetic_marker
AhTE0310AhTE0310genetic_marker
AhTE0311AhTE0311genetic_marker
AhTE0312AhTE0312genetic_marker
AhTE0313AhTE0313genetic_marker
AhTE0314AhTE0314genetic_marker
AhTE0315AhTE0315genetic_marker
AhTE0316AhTE0316genetic_marker
AhTE0317AhTE0317genetic_marker
AhTE0318AhTE0318genetic_marker
AhTE0319AhTE0319genetic_marker
AhTE0320AhTE0320genetic_marker
AhTE0321AhTE0321genetic_marker
AhTE0322AhTE0322genetic_marker
AhTE0323AhTE0323genetic_marker
AhTE0324AhTE0324genetic_marker
AhTE0325AhTE0325genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055