Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0276AhTE0276genetic_marker
AhTE0277AhTE0277genetic_marker
AhTE0278AhTE0278genetic_marker
AhTE0279AhTE0279genetic_marker
AhTE0280AhTE0280genetic_marker
AhTE0281AhTE0281genetic_marker
AhTE0282AhTE0282genetic_marker
AhTE0283AhTE0283genetic_marker
AhTE0284AhTE0284genetic_marker
AhTE0285AhTE0285genetic_marker
AhTE0286AhTE0286genetic_marker
AhTE0287AhTE0287genetic_marker
AhTE0288AhTE0288genetic_marker
AhTE0289AhTE0289genetic_marker
AhTE0290AhTE0290genetic_marker
AhTE0291AhTE0291genetic_marker
AhTE0292AhTE0292genetic_marker
AhTE0293AhTE0293genetic_marker
AhTE0294AhTE0294genetic_marker
AhTE0295AhTE0295genetic_marker
AhTE0296AhTE0296genetic_marker
AhTE0297AhTE0297genetic_marker
AhTE0298AhTE0298genetic_marker
AhTE0299AhTE0299genetic_marker
AhTE0300AhTE0300genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055