Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0251AhTE0251genetic_marker
AhTE0252AhTE0252genetic_marker
AhTE0253AhTE0253genetic_marker
AhTE0254AhTE0254genetic_marker
AhTE0255AhTE0255genetic_marker
AhTE0256AhTE0256genetic_marker
AhTE0257AhTE0257genetic_marker
AhTE0258AhTE0258genetic_marker
AhTE0259AhTE0259genetic_marker
AhTE0260AhTE0260genetic_marker
AhTE0261AhTE0261genetic_marker
AhTE0262AhTE0262genetic_marker
AhTE0263AhTE0263genetic_marker
AhTE0264AhTE0264genetic_marker
AhTE0265AhTE0265genetic_marker
AhTE0266AhTE0266genetic_marker
AhTE0267AhTE0267genetic_marker
AhTE0268AhTE0268genetic_marker
AhTE0269AhTE0269genetic_marker
AhTE0270AhTE0270genetic_marker
AhTE0271AhTE0271genetic_marker
AhTE0272AhTE0272genetic_marker
AhTE0273AhTE0273genetic_marker
AhTE0274AhTE0274genetic_marker
AhTE0275AhTE0275genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055