Overview
TitleCharacterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome
AuthorsShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S
TypeJournal
Media TitleTheoretical and Applied Genetics
Volume124
Issue8
Year2012
Page(s)1429-1438
CitationShirasawa K, Hirakawa H, Tabata S, Hasegawa M, Kiyoshima H, Suzuki S, Sasamoto S, Watanabe A, Fujishiro T, Isobe S. (2012). Characterization of Active Miniature Inverted-Repeat Transposable Elements in the Peanut Genome. Theoretical and Applied Genetics. 124:8 1429-1438

Abstract

Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives.
Features
This publication contains information about 504 features:
Feature NameUniquenameType
AhTE0026AhTE0026genetic_marker
AhTE0027AhTE0027genetic_marker
AhTE0028AhTE0028genetic_marker
AhTE0029AhTE0029genetic_marker
AhTE0030AhTE0030genetic_marker
AhTE0031AhTE0031genetic_marker
AhTE0032AhTE0032genetic_marker
AhTE0033AhTE0033genetic_marker
AhTE0034AhTE0034genetic_marker
AhTE0035AhTE0035genetic_marker
AhTE0036AhTE0036genetic_marker
AhTE0037AhTE0037genetic_marker
AhTE0038AhTE0038genetic_marker
AhTE0039AhTE0039genetic_marker
AhTE0040AhTE0040genetic_marker
AhTE0041AhTE0041genetic_marker
AhTE0042AhTE0042genetic_marker
AhTE0043AhTE0043genetic_marker
AhTE0044AhTE0044genetic_marker
AhTE0045AhTE0045genetic_marker
AhTE0046AhTE0046genetic_marker
AhTE0047AhTE0047genetic_marker
AhTE0048AhTE0048genetic_marker
AhTE0049AhTE0049genetic_marker
AhTE0050AhTE0050genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Speciesarachis
URLhttp://link.springer.com/article/10.1007/s00122-012-1798-6
KeywordsNULL
Cross References
This publication is also available in the following databases:
DatabaseAccession
DOI: Document Object IdentifierDOI:10.1007/s00122-012-1798-6
PMID: PubMedPMID:3336055